The kidney controls the volume and composition of extracellular fluid and participates in the regulation of blood pressure. Its regulatory function can be understood in terms of the action of resident vascular and epithelial cells. The functional unit of the kidney is the nephron, a long epithelial tubule with attendant vasculature. The kidney processes blood in two basic steps: (1) an ultrafiltrate of blood plasma is formed in specialized vascular capillaries and this fluid enters the renal tubule; (2) the renal tubules transform the ultrafiltrate into urine by means of differential transport of solutes and water through the tubule epithelial cells. These two processes influence each other: the ultrafiltration rate impacts tubule function, and tubule transport can modulate ultrafiltration. Both processes are influenced by body fluid composition, and by neural and hormonal signals that impact on the kidney.
The workshop will focus on the application of mathematical models to elucidate renal function in the context of new experimental methods and data. Physiologists, biophysicists, modelers, and mathematicians will present recent work and discuss current controversies and emerging issues. Topics may include: the regulation of ion channels in renal tubular cells, the regulation of renal hemodynamics, tubular-vascular interactions, new insights into the urine concentrating mechanism, new analytical methods, international computational initiatives, and web-based modeling resources.
Schedule |
Monday, February 19
Transport and Signaling |
| 8:30-8:45am |
Welcome: Avner Friedman |
| 8:45-9:15am |
Introduction: Harold Layton and Alan Weinstein |
| 9:15-10:00am |
Peter Jordan: Gating Ion Channels: the Interplay of Structure and Theory |
| 10:00-10:30am |
Coffee break |
| 10:30-11:15am |
Benoit Roux |
| 11:15-12:00pm |
Mark Schumaker: Framework models of ion permeation |
| 12:00-1:30pm |
Lunch break |
| 1:30-2:15pm |
Donald Loo: Conformational Dynamics of SGLT1 during Na+/glucose Cotransport |
| 2:15-3:00pm |
Ian Forster: Electrogenic phosphate transport across renal epithelia: mechanistic insights from experiments and simulations |
| 3:00-3:30pm |
Coffee break |
| 3:30-4:15pm |
Alan Weinstein |
| 5:00-7:00pm |
Reception in MW 724 |
Tueday, February 20
Transport and Signaling (continued) |
| 8:30-9:15am |
Thomas Pallone: Vasoactivity and ion channel architecture of the descending vasa recta wall |
| 9:15-10:00am |
Aurelie Edwards: Modification of Cytosolic Calcium Signaling by Subplasmalemmal Microdomain in Outer Medullary Pericytes |
| 10:00-10:30am |
Coffee break |
| 10:30-11:15am |
Sheldon Weinbaum: A new view of the Starling hypothesis and the role of pericytes in the temporal regulation of microvascular exchange |
| 11:15-12:15pm |
Roundtable symposium: "Modeling transporter function: at what scale, toward what end?" Alan Weinstein (moderator) |
| 12:15-1:45pm |
Lunch break |
| Renal Physiome |
| 1:45-2:30pm |
S. Randall Thomas: Overview of Physiome: QKDB, ontology needs, European & International funding |
| 2:30-3:00pm |
Peter Harris: The Virtual Kidney: Progress with a 3D anatomical interface, model repository and grid portal for distributed computing |
| 3:00-3:30pm |
Coffee break |
| 3:30-4:00pm |
Robert Moss: A Preliminary Model for Studying the Interactions Between Nephrons |
| 4:00-5:00pm |
Roundtable Symposium: "Big Kidney: The Renal Physiome Project"
Panel: Peter Harris, Leon Moore, and James Schafer (moderator) |
Wednesday, February 21
The Urine Concentrating Mechanism and Urea Transport |
| 8:30-9:15am |
William Dantzler: Three-dimensional functional reconstructions of vascular and tubular structures of inner medulla |
| 9:15-9:45am |
Thomas Pannabecker: Quantitative Analyses of Nephron and Blood Vessel Architecture in the Renal Inner Medulla |
| 9:45-10:15am |
Coffee break |
| 10:15-10:45am |
Erik Christensen: Reconstruction of the mouse nephron and distribution of AQP-1 and UT-A2 |
| 10:45-11:15am |
Lise Bankir: (i) Vascular-tubular relationships in a typical mammalian kidney; (ii) Movie by Reiner Beeuwkes (presented by Lise Bankir): The human kidney |
| 11:15-1:30pm |
Lunch and poster session: Lise Bankir, Saziye Bayram, Boubacar Benziane, Sylvain Demey, Paula B. Grajdeanu, Nicole Kleinstreuer, James A. Schafer, Kin Lung Siu, and Kay-Pong D. Yip |
| 1:30-2:00am |
Jeff Sands: Vasopressin regulation of the UT-A1 renal urea transporter |
| 2:00-2:30pm |
Alan Verkman: Chemical 'knock-out' by small-molecules to probe components of the urinary concentrating system |
| 2:30-3:00pm |
Mark Knepper: Analysis of cell signaling networks using proteomics methods and ODE-based modeling |
| 3:00-3:30pm |
Coffee break |
| 3:30-4:00pm |
Mariano Marcano: Optimization Problems and Algorithms for Mathematical Models of Renal Systems |
| 4:00-5:00pm |
Roundtable symposium: "Concentrating urine: Knowledge versus Understanding"
Panel: Lise Bankir, Rex Jamison (moderator), and Wilhelm Kriz |
Thursday, February 22
Renal Hemodynamics: Macula Densa Transport and Signal Transduction |
| 8:30-8:45am |
Leon Moore: General introduction |
| Macula Densa Transport and Signal Transduction: Volker Vallon, Chair |
| 8:45-9:20am |
Roland Blantz: The Tubular Vascular Relationship of TGF: Is there a Metabolic Contribution to this Connection? |
| 9:20-10:00am |
Jurgen Schnermann: NKCC2 Isoforms and TGF signaling |
| 10:00-10:30am |
Coffee break |
| 10:30-11:10am |
P. Darwin Bell |
| 11:10-12:00pm |
Roundtable symposium: "Macula densa models: Are we ready yet?" |
| 12:00-1:30pm |
Lunch break |
| Mechanisms of Renal Autoregulation: Bill Arenshorst and Branko Braam, Chair |
| 1:30-1:45pm |
Anil Bidani: Introduction and overview |
| 1:45-2:15pm |
Roger Loutzenhiser: Systolic Pressure is the Dominant Signal in Autoregulation |
| 2:15-2:45pm |
Geoffery Williamson: Simulation Studies of Dynamic Models for Renal Autoregulation |
| 2:45-3:15pm |
Coffee break |
| 3:15-3:45pm |
Armin Just: Evidence for a third and fourth regulatory mechanism in renal blood flow autoregulation |
| 3:45-4:15pm |
Ki Chon: Insights from Time-Varying Spectral Analysis |
| 4:15-5:00pm |
Roundtable symposium : "What's missing: Models and data" |
| 6:00-9:00pm |
Dinner at the Holiday Inn on the Lane |
Friday, February 23
Origins of Hemodynamic Complexity: Will Cupples and Suzanne Ditlevsen |
| 8:30-8:45am |
William Cupples: Introduction and overview |
| 8:45-9:15am |
Niels-Henrik Holstein-Rathlou: Synchronization among mechanisms of renal autoregulation is reduced in
hypertensive rats |
| 9:15-9:45am |
Donald Marsh: Routes to chaos in nephron blood flow regulation: nephron synchronization and ensemble formation |
| 9:45-10:15am |
Coffee break |
| 10:15-10:45am |
Anita Layton: Multistable Dynamics Mediated by Tubuloglomerular Feedback in a Model of Coupled Nephrons |
| 10:45-11:15am |
Harold Layton: Harmonics and heterodyning in the tubuloglomerular feedback loop |
| 11:15-11:45am |
Leon Moore: Sources of complexity in a model of the TGF system |
| 11:45-12:30pm |
Roundtable symposium |
